IDENTIFICATION OF LACTIC ACID BACTERIA OF THE GENUS LACTOBACILLUS, ISOLATED FROM SPONTANEOUS SOUR CLOTTED MILK AND INVESTIGATION OF THEIR ACID RESISTANECE AND BILE TOLERANCE
Abstract
PCR-analysis of the nucleotide sequences of the 16S rRNA gene was used to identify lactic acid bacteria, isolated from dairy products (sour clotted milk) from the Azerbaijan region of Iran. Eight strains were identified as Lactobacillus plantarum and seven strains - as Lactobacillus acidophylus. The analysis of acid and bile tolerance of the strains isolated from sour clotted milk showed that at pH 2.5 two strains were sensitive (10%-20% survival), 5 strains - tolerable (26%-48% survival), 6 strains - resistant (52%-76% survival) and 2 strains - more resistant (84%-86% survival). As for the bile tolerance, two strains were sensitive (10 min delay in growth), 3 strains were tolerant (20-30 min delay), 6 strains were resistant (40-50 min delay) and 4 strains were more resistant (60 min delay).
About the Authors
Х. Ганбаров
Бакинский государственный университет (Азербайджан)
Russian Federation
Russian Federation
Х. Моджаррад
Бакинский государственный университет (Азербайджан)
Russian Federation
Russian Federation
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