DESIGN OF OLIGONUCLEOTIDE PRIMERS FOR PCR-AMPLIFICATION OF INTERMICROSATELLITE DNA FRAGMENTS

Abstract

We consider the design of oligonucleotide primers for PCR. Those types of tests are described that can be performed using ISSR and RAPD methods: molecular analysis of DNA polymorphism and detection of individual, intra-population or species peculiarities of heredity. Microsatellites are encountered with high frequency in eukaryotic genomes. Microsatellite repetitions are often used in the study of DNA polymorphism. In order to achieve highly specific results in these studies the ends of oligonucleotide primers need to be consolidated with the help of selective nucleotides. The advantage of the variety of PCR analysis is the fact that the establishment of appropriate primers does not require prior knowledge of the nucleotide sequence of the target DNA.