Preview

Geographical Environment and Living Systems

Advanced search

EFFECT OF VARIOUS METHODS OF PRETREATMENT OF PLANT MATERIAL ON THE QUALITY OF GENOMIC DNA ISOLATED FROM IT

Abstract

We present the results of the experiments on the comparative evaluation of the efficiency of various methods for preservation of plant material until DNA isolation from it. The main criterion for this evalauation was applicability of obtained DNA samples for PCR amplification. Based on these results it was concluded that all tested method allowed one to obtain the DNA samples having good PCR applicability. However, from practical point of view the method for preservation of plant material in the form of conventional herbarium should be considered as the most optimal.

About the Authors

I. . Badulina
Moscow State Regional University
Russian Federation


V. . Martynov
Moscow State Regional University
Russian Federation


References

1. Звягин А.С. Выделение ДНК из гербарных листьев Vitis vinifera L. // Научный журнал КубГАУ. - 2010. - № 58. - С. 1-12.

2. Guidet. F. A powerful new technique to quickly prepare hundreds of plant extracts for PCR and RAPD analyses // Nucl. Acids Res. - 1994. - V. 22 (№ 9). - P. 1772-1773.

3. Junghans H., Metzlaff M. A simple and rapid method for the preparation of total plant DNA // BioTechniques. - 1990. - № 8. - P. 176.

4. Stewart C.N.Jr., Via L.E. A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications // BioTechniques. - 1993. - V. 14. - P. 748-749.


Review

Views: 86


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 2712-7613 (Print)
ISSN 2712-7621 (Online)