Preview

Geographical Environment and Living Systems

Advanced search

STUDY OF BACTERIOCINS PRODUCED BY THE STRAIN ENTEROCOCCUS FAECALIS T23 ISOLATED FROM IRANIAN CHEESE

Abstract

We have partially characterized the bacteriocin activity of the strain Enterococcus faecalis T23. The antimicrobial activity of this strain was determined against Listeria monocytogenes, Salmonella typhimurium and closely related Lactobacilli strains. Treatment with different enzymes revealed that the active substance has a protein nature. PCR amplification resulted in detection of two bacteriocin genes: entB and entA. Bacteriocins of the studied strain were heat stable and active over a wide range of pH (3-10). Triton X-20, Triton X-80, Triton X-100, в-mercaptoetanol, Na-EDTA, SDS and NaCl did not influence the bacteriocin activity.

About the Authors

T. . Mirhadi Zadi
Baku State University
Russian Federation


N. . Abdullayeva
Baku State University
Russian Federation


A. . Quliyev
Baku State University
Russian Federation


References

1. Aymerich T., Holo H., Hevarstein L., Hugas M., Garriga M., Nes I.F. Biochemical and genetic characterization of enterocin A from Enterococcus faecium, a new antilisterial bacteriocin in the pediocin family of bacteriocins // Applied and Environmental Microbiology. - 1996. - № 62. - P.1676-1682.

2. Batdorj B., Dalgalarrondo M., Choiset Y., Pedroche J., Métro F., Prévost H., Chobert J.-M., Haertlé T. Purification and characterization of two bacteriocins produced by lactic acid bacteria isolated from Mongolian airag // Journal of Applied Microbiology. -2006. - № 101. - P. 837-848.

3. Ben Belgacem Z., Abriouel H., Ben Omar N., Lucas R., Martinez-Canamero M., Galvez A., Manai M. Antimicrobial activity, safety aspects and some technological properties of bacteriocinogenic Enterococcus faecium from artisanal Tunisian fermented meat // Food Control. - 2010. - № 21. - P. 462470.

4. Cintas L.M., Casaus P., Holo H., Hernandez P.E., Nes I.F., Hevarstein L.S. Enterocins L50A and L50B, two novel bacteriocins from Enterococcus faecium L50, are related to staphylococcal hemolysins // Journal of Bacteriology. - 1998. - № 180. - P. 1988-1994.

5. De Vuyst L., Moreno M., Revets, H. Screening for enterocins and detection of hemolysin and vancomycin resistance in enterococci of different origins // International Journal of Food Microbiology. -2003. - № 84. - P. 299-318.

6. Du Toit M., Franz C., Dicks L., Holzapfel W. Preliminary characterization of bacteriocins produced by Enterococcus faecium and Enterococcus faecalis isolated from pig faeces // Journal of Applied Microbiology. - 2000. - № 88. - P. 482-494.

7. Gong H.S., Meng X.C., Wang H. Plantaricin MG active against Gram negative bacteria produced by Lactobacillus plantarum KLDS1 isolated from “Jiaoke”, a traditional fermented cream from China // Food Control., 2010, № 21. - P. 89-96

8. Gutrnrrez J., Criado R., Citti R., MartHn M., Herranz C., Nes I.F.,Cintas L.M., Hern6ndez P.E. Cloning, production and functional expression of enterocin P, a sec-dependent bacteriocin produced by Enterococcus faecium P13, in Escherichia coli // International Journal of Food Microbiology. - 2005. - № 103. - P. 239-250.

9. Leroy F., Foulqurn Moreno M.R., De Vuyst L. Enterococcus faecium RZS C5, an interesting bacteriocin producer to be used as a co-culture in food fermentation // International Journal of Food Microbiology. - 2003. - № 88. - P. 235-240.

10. Rehaiem A., Martinez B., Manai M., Rodriguez A. Production of enterocin A by Enterococcus faecium MMRA isolated from ‘Rayeb, a traditional Tunisian dairy beverage // Journal of Applied Microbiology. - 2009. - № 108. - P. 1685-1693.

11. Tagg J., Dajani A., Wannamaker L. Bacteriocins of Gram-positive bacteria // Bacteriological Reviews. - 1976. - № 40. - P. 722-756.

12. Weisburg W., Barns S., Pelletier D., Lane D. 16S ribosomal DNA amplification for phylogenetic study // Journal of Bacteriology. - 1991. - № 173. - P. 697-703.

13. Yamamoto Y., Togawa Y., Shimoska M., Okazaki M. Purification and characterization of novel bacteriocin produced by Enterococcus faecalis strain RJ-11 // Applied and Environmental Microbiology. - 2003. - № 69. - P. 5746-5753.


Review

Views: 57


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 2712-7613 (Print)
ISSN 2712-7621 (Online)