Application of bacterial cold shock proteins in biotechnology
https://doi.org/ 10.18384/2310-7189-2018-1-86-94
Abstract
Most of the achievements of modern biotechnology are related to microorganisms, which is explained by their diversity, the relative simplicity of their study and cultivation. This review is dedicated to the application of bacterial cold shock proteins in biotechnology. These proteins bind DNA and RNA and melt secondary structures formed in nucleic acids, which is important in bacteria adaptation to different kinds of stresses. Genes coding bacterial cold chock proteins are used for transformation of bacteria and higher plants, which lead to an increase in stress tolerance. Also, purified recombimnant bacterial cold shock proteins are used to increase efficiency of different molecular biology reactions in vitro. In this review, the approaches applied in relevant studies are described in detail and their results are summarized.
About the Authors
Nikolai E. Zlobin
All-Russia Research Institute of Agricultural Biotechnology, Russian Academy of Sciences, Russia
Russian Federation
Russian Federation
Vasiliy V. Taranov
All-Russia Research Institute of Agricultural Biotechnology, Russian Academy of Sciences, Russia
Russian Federation
Russian Federation
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