BIOTECHNOLOGY OF OBTAINING A BIOLOGICALLY ACTIVE CONCENTRATE FROM FRUITS OF HIPPOPHAE RHAMNOIDES L
https://doi.org/ 10.18384/2310-7189-2018-3-138-147
Abstract
The chemical composition of the sea buckthorn fruit grown in Azerbaijan has been studied and the biotechnology of obtaining biologically active on the basis of its fruits has been developed. It has been established that the fruits of sea buckthorn are rich in biologically active substances: vitamins C and E, carotenes, polyphenols (catechins, flavonoids), organic acids, etc. The effect of the pectolytic enzyme on juice yield and viscosity of juice has been studied. It has been found that the most optimal condition for fermentation is 1.5 hours with the dosage of the enzyme preparation of 2.2 unit PcA/g of pectin. In this case, the yield of juice from the fruits of sea buckthorn increases to 33%, and the viscosity decreases by 85%. It has been revealed that in enzymatic treatment of sea buckthorn fruits the amount of dry substances, sugar, organic acids, polyphenols, catechins, flavonoids, and vitamin C increased by 1.4, 1.5, 1.4, 1.6, 1.7, 1.4, 1.3 times, respectively. The resulting enzyme juice was concentrated to a dry matter content of 40-45% in vacuum. The concentrate can be used as a preventive and common-restorative means and food additive.
About the Authors
Eldar Novruz Novruzov
Institute of Botany, National Academy of Sciences of Azerbaijan
Russian Federation
Russian Federation
Latafat Ahad Mustafaeva
Institute of Botany, National Academy of Sciences of Azerbaijan
Russian Federation
Russian Federation
Aydan Mirza Zeynalovа
Institute of Botany, National Academy of Sciences of Azerbaijan
Russian Federation
Russian Federation
References
1. Государственная фармакопея СССР. Вып. 2: Общие методы анализа. Лекарственное растительное сырье / 11-е изд., доп. М.: Медицина, 1990. 390 с.
2. Запрометов М.Н. Основы биохимии фенольных соединений. М.: Высшая школа, 1974. 212 с.
3. Макарова М.И., Макаров В.Г., Станкевич Н.М., Ермаков С.Б., Яшакина И.А. Характеристика антирадикальной активности экстрактов из растительного сырья и соединение в них // Растительные ресурсы. 2005. Т. 41, № 2. С. 106-115.
4. Методы биохимического исследования растений / Под ред. А.И. Ермакова; 3-е изд., перераб. и доп. Л.: Агропромиздат, 1987. 430 с.
5. Новрузов Э.Н. Антиоксидантная свойства флавоноидов сафлора // Биоантиоксидант: сборник трудов V международной конф. М.: Последнее слово, 1998. С. 68-69.
6. Новрузов Э.Н. Перспективные пищевые красильные растения Азербайджана // Труды Первой Всероссийской конференции по ботаническому ресурсоведению. СПб.: Ботанический институт им. В.Л. Комарова РАН, 1996. С. 202-203.
7. Новрузов Э.Н. Пигменты репродуктивных органов растений и их значение. Баку: Элм, 2010. 309 с.
8. Новрузов Э.Н., Исмаилов Н.М., Мамедов С.Ш. Фенольные соединения листьев Hippophae rhamnoides L. в Азербайджанской ССР // Растительные ресурсы. 1983. Т. 19, № 5. С. 354-356.
9. Петреченко М.В., Сухикина Т.В., Фурса Н.С. Спектрофотометрический метод определения содержание флавоноидов в Euphorbia brevepila Burm. et Gremli // Растительные ресурсы. 2002. Т. 38, № 2. С. 104-109.
10. Пищевая химия / Под ред. А.П. Нечаева. СПб.: ГИОРД, 2007. 640 с.
11. Шабров А.В., Дадали В.А., Макаров В.Г. Биохимические основы действия микрокомпонентов пищи. М.: Авваллон, 2003. 184 с.
12. Chai Q., Xiayan G., Jhao M. et al. The experimental studies on the cardiovascular pharmacology of seabuckthorn extract from Hippophae rhamnoides L. // Proceedings of the International Symposium on Seabuckthorn, Xi’an, China, 1989. Pр. 392-397.
13. Edenharder R., von Petersdorfi J., Baucher R. Antimutagenic effects of flavonoids, chalcones and structurally related compounds on the activity of 2-amino-3-methyl imidase (4,5-guinoline) (IQ) and other heterocyclic amine mutagens from cooked food // Mutat. Res., 1993, v. 287. Pp. 261-274.
14. Gryglewski R., Korbut R., Robak J., Swies J. On the mechanism of antithrombotic action of flavonoids // Biochemical Paharmacology, 1987, v. 36. Pp. 317-322.
15. Hertog M., Kromkout D., Aravanics C. Flavonoid intake and long-term risk of coronary heart disease and cancer in the seven countries study // Arch. Intern. Med., 1995, v. 155. Pp. 381-386.
16. Junior A., Asad L., Oliverra E. et al. Antigenetoxic and antimutagenic potential of an annato-pigment (norbixin) against oxidative stress // Genet. Mol. Res., 2005, v. 31, no. 4 (1). Pp. 94-109.
17. Novruzov E., Shamsizade L. The technology of processing of flowers of the saffron without waste // Proc. of 1st Inter. Symp. on Saffron biology and biotechnology, Spain, 2003, pp. 108.
18. Plant flavonoids in biology and medicine / Ed. by Cody V., Middleton E., Harborne J. New-York: Aban R. Liss, 1988, 461 p.
19. Ross R. The pathogenesis of atherosclerosis - an update // N. Eng. J. Med., 1986, v. 314. Pp. 488-500.
20. Swain T., Hillis W. The phenolic constituents of Prunus domestica I The quantitative analysis of phenolic constituents // J. Sci. Food. Agric., 1959, v. 10, no. 1. Pp. 63-70.
21. Tolkachev O., Shipulina L. Antiviral polyphenols from seabuckthorn leaves as the source of drug Hippomarin // Proc. of 1st Congr. of ISA, Berlin, 2003. Pp. 90-103.
22. Wu Y., Wang X., Wang R., Yang Y. Effects of total flavones of fruits of Hippophae (TFH) on cardiac function and hemodinamics of anesthetized open chest dogs with acute health failure // Zhangguo Jhonggao Zarzhi, 1997, v. 22, no. 7. Pp. 429-482.
23. Zhao F., Dieter S., Alfred B. et al. Antioxidant flavonoids from leaves of Polygonum hydropiper L. // Phytochemistry, 2003, v. 62, no. 2. Pp. 219-228.
Review
Views:
81
Email this article 