USE OF MODERN STERILIZATION AGENTS FOR IN VITRO GARLIC CULTURE (ALLIUM SATIVUM L.)
https://doi.org/10.18384/2712-7621-2020-4-120-128
Abstract
Aim. The purpose is to study the effectiveness of various disinfectants for the sterilization of the garlic plant material under introduction in vitro culture. Methodology. Sterilization of the plant material was carried out according to a two-stage scheme. Chlorine-containing and quaternary ammonium compounds, as well as water-soluble ‘Glavchlor’ granules and concentrated liquid preparations ‘Deo-bakter’ and ‘Megacid’ were used. The preparations were used in a concentration from 1% to 3% of the active substance and for an exposure time of 20 minutes. Results. Studies have shown that the preparation ‘Glavchlor’ when sterilizing the plant material of garlic, allows one to obtain up to 90% of infection-free explants, and the drug ‘Megacid’ can reduce the level of contamination by 7% - 29%. The use of the drug ‘Deo-bakter’ proved to be ineffective. Research implications. It is proved that ‘Glavchlor’ can be used as an alternative means for sterilization of plant material under introduction in vitro culture.
About the Authors
I. V. Muravyova
All-Russian Research Institute of Vegetable Production, Branch of the Federal State Budgetary Scientific Institute “Federal Scientific Center of Vegetable Production”
Russian Federation
Russian Federation
M. A. Azopkova
All-Russian Research Institute of Vegetable Production, Branch of the Federal State Budgetary Scientific Institute “Federal Scientific Center of Vegetable Production”
Russian Federation
Russian Federation
A. V. Polyakov
All-Russian Research Institute of Vegetable Production, Branch of the Federal State Budgetary Scientific Institute “Federal Scientific Center of Vegetable Production”; Moscow Region State University
Russian Federation
Russian Federation
References
1. Методология биотехнологических исследований цветочно-декоративных культур / под ред. И. В. Митрофановой. Симферополь: Ариал, 2018. 265 с.
2. Поляков А. В., Азопкова М. А., Муравьёва И. В. Получение in vitro посадочного материала чеснока озимого (Allium sativum L.) : Методическое руководство. М.: Всероссийский научно-исследовательский институт овощеводства - филиал федерального государственного бюджетного научного учреждения Федеральный научный центр овощеводства, 2018. 12 с.
3. Поляков А. В. Получение регенерантов овощных культур и их размножение in vitro // Методические рекомендации. М.: Всероссийский научно-исследовательский институт овощеводства Российской сельскохозяйственной академии. 2005. 36 с.
4. Прошлое, настоящее и будущее четвертичных аммонийных соединений. Дезинфектология на современном этапе / Опарин П. С., Тюрнева Н. А., Шептунов С. И., Опарина Т. П., Антонива Т. А., Панова М. А. Иркутск: Восточно-Сибирский научный центр Сибирского отделения Российской академии медицинских наук, 2003. 15 с.
5. Саттон Д., Фотeргилл А., Ринальди М. Определитель патогенных и условно патогенных грибов. М.: Мир, 2001. 464 с.
6. Ashwini P. Benke, Punam Shelke, V. Mahajan. Three step protocol for regeneration of plantlets in INDIAN Garlic varieties using root meristem. In: Indian Journal Of Agricultural Research, 2018, vol. 52(1), pp. 66-70.
7. Gull I. Comparative effect of different pyhtohormones on the micropropagation of Allium sativum. In: Pak. J. Biochem. Mol. Biol, 2014, vol. 47(1-2), pp. 121-124.
8. Metwally E. I. In vitro propagation of garlic (Allium sativum L.) trough adventitioses shoot organogenesis. In: African Jornal of Biotechnolog, 2014, vol. 13, no. 38, pp. 3892-3900.
9. Scotton D. S., Benedito V. A., Molfetta J. B., Rodrigues B. I. Fp., Tulmann-Neto A., Figueira A. Response of root explants to in vitro cultivation of marketable garlic cultivars. In: Horticultura Brasiliera, 2013, vol. 31, no. 1, pp. 80-85.
10. Tomaszewska-Sowa M., Figas A., Keutgen N., Keutgen A. J. Establishing an efficient explant superficial sterilization protocol for in vitro micropropagation of bear’s garlic (Allium ursinum L.). In: Herba Polonica, 2015, vol. 61(4), pp. 66-77.
Review
Views:
82
Email this article 