SINGLE AND COMBINED EFFECTS OF HYPOXIA/ANOXIA AND COPPER ON ANTIOXIDANT SYSTEM OF LITTORINA MANDSCHURICA
Abstract
The single and combined effects of hypoxia/
anoxia and copper on antioxidant system of Littorina
mandschurica were evaluated under laboratory
conditions. Activities superoxide dismutase, catalase,
glutathione reductase, glutathione S-transferase, the
levels of glutathione, total antioxidant activity (ABTS
method) were assayed in hepatopancreas. Peroxidative
damage level was assessed by determining malondialdehyde.
The results showed that combined effects of
hypoxia/anoxia and copper increase oxidative stress in
molluscs.
anoxia and copper on antioxidant system of Littorina
mandschurica were evaluated under laboratory
conditions. Activities superoxide dismutase, catalase,
glutathione reductase, glutathione S-transferase, the
levels of glutathione, total antioxidant activity (ABTS
method) were assayed in hepatopancreas. Peroxidative
damage level was assessed by determining malondialdehyde.
The results showed that combined effects of
hypoxia/anoxia and copper increase oxidative stress in
molluscs.
About the Authors
Александра Истомина
Учреждение Российской академии наук Тихоокеанский
океанологический институт им. В.И. Ильичева
Дальневосточного отделения РАН
Russian Federation
Russian Federation
Надежда Довженко
Учреждение Российской академии наук Тихоокеанский
океанологический институт им. В.И. Ильичева
Дальневосточного отделения РАН
Russian Federation
Russian Federation
Нина Бельчева
Учреждение Российской академии наук Тихоокеанский
океанологический институт им. В.И. Ильичева
Дальневосточного отделения РАН
Russian Federation
Russian Federation
Виктор Челомин
Учреждение Российской академии наук Тихоокеанский
океанологический институт им. В.И. Ильичева
Дальневосточного отделения РАН
Russian Federation
Russian Federation
References
1. Биргер Т.И. Метаболизм водных беспозвоночных в токсической среде. Киев: Наук. Думка, 1979. 192 с.
2. Ланкин В.З., Тихазе А.К., Беленков Ю.Н. Свободнорадикальные процессы в норме и при патологических состояниях: пособие для врачей. М.: РКНПКМЗ РФ, 2001. 78 с.
3. Челомин В.П., Бельчева Н.Н., Довженко Н.В. Мониторинг загрязнения прибрежных вод на основе биохимических маркеров. В кн.: Дальневосточные моря России. Исследования морских экосистем и биоресурсов. М.:Наука, 2007. с. 617- 632.
4. Челомин В.П., Бельчева Н.Н., Захарцев М.В. Биохимические механизмы адаптации мидии Mytilus trossulus к ионам кадмия и меди // Биология моря. 1998, ¢5. С. 319-325.
5. Buege J.A., Aust S.D. Microsomal lipid peroxidation. Methods in Enzymology, Eds. by Fleischer S., Packer L., N.Y.: Academic Press. 1978. P. 302-310.
6. Chen M., Yang H., Delaporte M., Zhao S., Xing K. Immune responses of the scallop Chlamys farreri after air exposure to different temperatures // Journal of Experimental Marine Biology and Ecology. 2007. V. 345. P. 52-60.
7. Fang J.K.H., Wu R.S.S., Chan A.K.Y., Yip C.K.M., Shin P.K.S. Influences of ammonia-nitrogen and dissolved oxygen on lysosomal integrity in greenlipped mussel Perna viridis: Laboratory evaluation and field validation in Victoria Harbour, Hong Kong // Marine Pollution Bulletin. 2008. V. 56. P. 2052-2058.
8. Freedman J.Н., Ciriolo M.R, Peisach J. The role of glutathione in copper metabolism and toxicity // The journal of biological chemistry. 1989. Vol. 264. No.10. P. 5598-5605.
9. Habig, W.H., Jakoby W.B. Assay for differentiation of glutathione S-transferases // Meth. Enzymol. 1981. V. 77. P. 398-405.
10. Halliwell B., Gutteridge J.M.C. Free radicals in biology and medicine. Oxford: Oxford Univ. press, 2007. 851 p.
11. Hermes-Lima M., Storey J.M., Storey K.B. Antioxidant defenses and metabolic depression. The hypothesis of preparation for oxidative stress in land snails // Comparative Biochemistry and Physiology. 1998. Part B 120. P. 437-448.
12. Lowry O.H., Rosenbrough N.J., Farr A.L. and Randall R.J. Protein measurement with the folin phenol reagent // J. Biol. Chem. 1951. V. 193. P. 266-275.
13. Moron M.S., Depierre J.W., Mannervik B. Levels of glutathione, glutathione reductase and glutathione stransferase activities in rat lung and liver // Biochim. Biophys. Acta. 1979. V. 582. P. 67-78.
14. Paoletti F., Aldinuccio D., Mocali A., Carparrini A. A sensitive spectrophotometric method for the determination of superoxide dismutase in tissue extracts // Anal. Biochem. 1986. V. 154. P. 526-541.
15. Regoli F., Principato G. Glutathione, glutathionedependent and antioxidant enzymes in mussel, Mytilis galloprovincialis, exposed to metals under field and laboratory conditions: implications for the use of biochemical biomarkers // Aquat. Toxicol. 1995. Vol. 31. P. 143-164.
16. Re Roberta, Pellegrini N., Proteggente A., Pannala A., Yang M. and Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay // Free Radical Biology & Medicine. 1999. Vol. 26. P. 1231-1237.
17. Rudolf S.S. Wu. Hypoxia: from molecular responses to ecosystem responses // Marine Pollution Bulletin. 2002. V. 45. P. 35-45.
18. Valko M., Morris H., Cronin M.T.D. Metals, Toxicity and Oxidative Stress // Current Medicinal Chemistry. 2005. Vol. 12. P. 1161-1208.
19. Veldhuizen-Tsoerkan M.B. Anoxic survival time and metabolic parameters as stress indices in sea mussels exposed to cadmium or polychlorinated biphenyls // Archives of Environmental Contamination and Toxicology. 1991. V. 20. P. 259-265.
20. Wilson J.G., McMahon R.F. Effects of high environmental copper concentration on the oxygen consumption, condition and shell morphology of natural populations of Mytilus edulis L. and Littorina rudis Maton // Comp. Biochem. Physiol. 1981. V. 70. P. 139-147.
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