IDENTIFICATION AND CHARACTERIZATION OF MIPS1 GENE IN THE GENUS GLYCINE
Abstract
Based on the literature data and the study of nucleotide database of NCBI, the part of gene MIPS1, involved in the biosynthesis of phytic acid, was PCR amplified in plants belonging to the genus Glycine. The resulting amplicons (15 amplicons) were cloned and sequenced. Analysis of the sequences showed that they all belong to a gene MIPS1 in species Glycine max, G. soja and G. gracilis. Comparative analysis of obtained nucleotide sequences revealed a polymorphism of their primary structure. Furthermore, data about the nucleotide sequence of gene MIPS1 and its polymorphism in species G. soja and G. gracilis were obtained for the first time.
About the Authors
В. Мартынов
Центр «Биоинженерия» РАН
Russian Federation
Russian Federation
Д. Дорохов
Московский государственный областной университет
Russian Federation
Russian Federation
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