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EFFECT OF PROTEASES FROM STRAIN ENTEROCOCCUS FAECALIS AN1 ON LIBERATION OF ANTIMICROBIAL PEPTIDES FROM CASEINS AND THEIR STABILITY IN THE IN VITRO SIMULATED GASTROINTESTINAL SYSTEM

Abstract

The aim of the present research was to study
the effect of proteolytic strain Enterococcus faecalis AN1 on
liberation of casein-derived antimicrobial peptides, as well as
also to determine the infl uence of the subsequent hydrolysis
by pepsin and pancreatin in the in vitro stimulated gastrointestinal
system on this activity. The protein hydrolysis and
peptide production were analyzed with the help of SDS PAGE
and RP-HPLC. The peptides released upon hydrolysis of
caseins with the studied strain presented antimicrobial activity
against Listeria monocytogenes EGDe107776. Antimicrobial
activity of casein-derived peptides was stable after pepsin
hydrolysis; however, the effect totally disappeared after
pancreatic digestion. Moreover, prehydrolysis of caseins by
proteases from strain Enterococcus faecalis AN1 increased
their digestion by pepsin. The strain under study could be a
potential starter for production of functional dairy products.

About the Authors

Р. Мустафаева
Бакинский государственный университет
Russian Federation


С. Гюльахмедов
Бакинский государственный университет
Russian Federation


А. Кулиев
Бакинский государственный университет
Russian Federation


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ISSN 2712-7613 (Print)
ISSN 2712-7621 (Online)