BIOSYNTHESIS OF LIPIDS IN TWO OIL-DEGRADING FUNGI - CEPHALOSPORIUM HUMICOLA AND MUCOR CLOBOSUS
Abstract
We study the effect of the carbon sources - glucose
and hexadecane - on the fatty acid composition and
activity of enzymes, actually or potentially involved in the biochemistry
of oleaginicity of two oil degrading fungi, namely, Cephalosporium humicola and Mucor globosus. The carbon
sources markedly affect the fatty acid composition of the fungi
in question. The lipids extracted from hexadecane grown cells
contain not only saturated fats found in fungi grown on glucose
substrates but also linoleic and linolenic acids. In both
fungi we observe a dramatic decrease in the stearic acid
content from 16,0-29.0% to 2,8-5.9% by replacing glucose by
hexadecane. The synthase activity of fatty acids is found in
cells growing both on glucose and hexadecane substrates.
Isocitrate lyase is absent in cells growing on glucose substrates
and is found when grown on hexadecane substrates.
The activity of adenosine triphosphate is as follows: citrate
lyase is completely inhibited by hexadecane. Carnitine acetyltransferase
is detected in cells grown on glucose substrates.
The activity of this enzyme increases tenfold in the case when
hexadecane is the source of carbon. The high activity of pyruvate
kinase on glucose decreases by 80%-90% when grown
on a hydrocarbon substrate. Analysis of the results and products
of linoleic and linolenic acids shows that the hydrocarbon
substrate increases the biosynthesis of 18:2 and 18:3 acids,
and, hence, 12/15-desaturase enzymes, which are responsible
for the conversion of 18:1-to-18:2 and 18:2-to-18:3
acids. Although the specific activities of the enzymes vary between
the fungi, the developmental profiles for all enzymes
are virtually similar.
and hexadecane - on the fatty acid composition and
activity of enzymes, actually or potentially involved in the biochemistry
of oleaginicity of two oil degrading fungi, namely, Cephalosporium humicola and Mucor globosus. The carbon
sources markedly affect the fatty acid composition of the fungi
in question. The lipids extracted from hexadecane grown cells
contain not only saturated fats found in fungi grown on glucose
substrates but also linoleic and linolenic acids. In both
fungi we observe a dramatic decrease in the stearic acid
content from 16,0-29.0% to 2,8-5.9% by replacing glucose by
hexadecane. The synthase activity of fatty acids is found in
cells growing both on glucose and hexadecane substrates.
Isocitrate lyase is absent in cells growing on glucose substrates
and is found when grown on hexadecane substrates.
The activity of adenosine triphosphate is as follows: citrate
lyase is completely inhibited by hexadecane. Carnitine acetyltransferase
is detected in cells grown on glucose substrates.
The activity of this enzyme increases tenfold in the case when
hexadecane is the source of carbon. The high activity of pyruvate
kinase on glucose decreases by 80%-90% when grown
on a hydrocarbon substrate. Analysis of the results and products
of linoleic and linolenic acids shows that the hydrocarbon
substrate increases the biosynthesis of 18:2 and 18:3 acids,
and, hence, 12/15-desaturase enzymes, which are responsible
for the conversion of 18:1-to-18:2 and 18:2-to-18:3
acids. Although the specific activities of the enzymes vary between
the fungi, the developmental profiles for all enzymes
are virtually similar.
About the Authors
М. Гасеми
Институт микробиологии НАН Азербайджана (г. Баку)
Russian Federation
Russian Federation
Я. Атакишиева
Институт микробиологии НАН Азербайджана (г. Баку)
Russian Federation
Russian Federation
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