LOVASTATIN PRODUCTION BY ASPERGILLUS TERREUS ON DIFFERENT SUBSTRATES IN SOLID-PHASE FERMENTATION
Abstract
We report the possibility of solid-phase fermentation of local strains A.terreus 4 and A.terreus 20 on different substrates with a significant yield of lovastatin. The greatest growth of producers and maximum accumulation of statins in both strains on all the substrates is observed on the 11 day of fermentation. The level of lovastatin production in the strain A.terreus 4 is higher than in A.terreus 20. It is found that the level of biosynthesis of lovastatin in A.terreus 4 when using oats as a solid substrate considerably exceeds all other substrates used. The maximum quantity is equal to 4.32 mg/g of dry substrate when the methanol extraction is employed.
About the Authors
D. . Ruzieva
Uzbekistan Academy of Science, Microbiology Institute
Russian Federation
Russian Federation
G. . Rasulova
Uzbekistan Academy of Science, Microbiology Institute
Russian Federation
Russian Federation
K. . Lobanova
Uzbekistan Academy of Science, Microbiology Institute
Russian Federation
Russian Federation
F. . Karimova
Uzbekistan Academy of Science, Microbiology Institute
Russian Federation
Russian Federation
T. . Gulyamova
Uzbekistan Academy of Science, Microbiology Institute
Russian Federation
Russian Federation
References
1. ГОСТ 15113.4-77: межгосударственный стандарт. Концентраты пищевые. Методы определения влаги / Федеральное агентство по техническому регулированию и метрологии [сайт]. URL: http://pro-tect.gost.ru/document.aspx?control=7&id=158130 (дата обращения 17.04.2013).
2. Adinarayana K. Optimization of process parameters for cephalosporin C production under solid state fermentation from Acremonium chrysogenum / K. Adinarayana, T. Prabhakar, V. Srinivasulu et al. // Process Biochem. -2003. - V. 39 (№ 2). - P. 171-177.
3. Banos J.G. High lovastatin production by Aspergillus terreus in solid-state fermentation on polyurethane foam: An artificial inert support / J.G. Banos, A. Tomasini, G. Szakacs et al. // J. of Bioscience and Bioenineering. - 2009. - V. 108 (№ 2). - P. 105-110.
4. Casas Lopez J.L. Pellet morphology, culture rheology and lovastatin production in cultures of Aspergillus terreus / Casas Lopez J.L., Sanchez Perez J.A., Fernandez Sevilla J.M. et al. // J. of Biotechnol. - 2005. - № 116. - P. 61-77.
5. Gombert A.K. Lipase production by Penicillium restrictum in solid state fermentation using babassu oil cake as substrate / A.K. Gombert, A.L. Pinto, L.R. Astilho et al. // Process Biochem. - 1999. - V. 35 (№ 1-2). - P. 85-90.
6. Ramana Murthy M.V., Mohan E.V.S., Sadhukhan A.K. Cyclosporin-A production by Tolypociadium inflatum using solid stste fermentation // Process Biochem. - 1999. - V 34 (№ 3). - P. 269-280.
7. Wei Pi-lian, Xu Zhi-nan, Cen Рei-lin. Lovastatin production by aspergillus terreus in solid-state fermentation // J. of Zhejiang University SCIENCE A. - 2007. - V. 8 (№ 9). - P. 1521-1526.
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