ANALISIS OF GROWTH OF ARABIDOPSIS THALIANA MUTANTS WITH REDUCED RATE OF CYTOKININ SYNTHESIS
Abstract
We report an example of applying the method for analyzing root growth at a cellular level in Arabidopsis thaliana mutants with reduced cytokinin concentration in roots due to impaired expression of the genes encoding the enzyme ATP/ADP isopentenyl transferase which catalyses the first reaction of cytokinin biosynthesis pathway, as well as in wild type plants. We prove the possibility of using this mutant to unravel the role of cytokinins in the regulation of cell cycle duration. A formula for calculating cell cycle duration is also provided.
About the Author
A. . Filin
Timiryazev Institute of Plant Physiology, Russian Academy of Sciences
Russian Federation
Russian Federation
References
1. Иванов В.Б. Клеточные основы роста растений. - М.: Наука, 1974. -223 с.
2. Beemster G.T.S., Baskin T.I. STUNTED PLANT 1 Mediates Effects of Cytokinin, But not of Auxin, on Cell Division and Expansion in the Root of Arabidopsis // Plant Physiology. - 2000. - Vol. 124 (№ 4). - P. 1718-1727.
3. Casamitjana-Martinez E. Root-Specific CLE19 Overexpression and the sol1/2 Suppressors Implicate a CLV-like Path way in the Control of Arabidopsis Root Meristem Maintenance / E. Casamitjana-Martinez, H.F. Hofhuis, Jian Xu et al. // Current Biology. - 2003. - Vol. 13 (№ 16). - P. 1435-1441.
4. Ioio R. D. Cytokinins Determine Arabidopsis Root-Meristem Size by Controlling Cell Differentiation / R.D. Ioio, F.S. Linhares, E. Scacchi et al. // Current Biology. - 2007. - Vol. 17 (№ 8). - P. 678-682.
5. Ivanov V.B., Dubrovsky J.G. Estimation of the cell-cycle duration in the root apical meristem: a model of linkage between cell-cycle duration, rate of cell production, and rate of root growth // International J. of Plant Sciences. - 1997. - Vol. 158 (№ 6). - P. 757-763.
6. Satbhai S.B., Ristova D., Busch W. Underground tuning: quantitative regulation of root growth // J. of Experimental Botany. - 2015. - Vol. 66 (№ 4). - P. 1099-1112.
Review
Views:
62
Email this article 