geomgouГеографическая среда и живые системыGeographical Environment and Living Systems2712-76132712-7621Московский государственный областной университетgeomgou-911Research ArticleСтатьиРЕАКЦИЯ АНТИОКСИДАНТНОЙ СИСТЕМЫ НА АНОКСИЮ И РЕОКСИГЕНАЦИЮ У МОРСКОГО ДВУСТВОРЧАТОГО МОЛЛЮСКА SCAPHARCA BROUGHTONIANTIOXIDANT DEFENSES DURING ANOXIA AND AEROBIC RECOVERY IN MARINE BIVALVIA SCAPHARCA BROUGHTONIИстоминаАлександра Анатольевнаs-istomina1@mail.ruДовженкоНадежда Владимировнаs-istomina1@mail.ruЧеломинВиктор Павловичs-istomina1@mail.ruТихоокеанский океанологический институт им. В.И. Ильичева (Владивосток)Russian Federation201027052022043943Copyright © Истомина А.А., Довженко Н.В., Челомин В.П., 20222022Истомина А.А., Довженко Н.В., Челомин В.П.Истомина А.А., Довженко Н.В., Челомин В.П.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.geoecosreda.ru/jour/article/view/911

В работе рассматриваются особенности биохимической организации и реакции антиоксидантной (АО) защитной системы в тканях пищеварительной железы и жабр моллюска Scapharca broughtoni в условиях экспериментальной аноксии/гипоксии с последующей реоксигенацией. Полученные результаты выявили, что аноксия вызвала общее подавление АО активности, снижение уровня глутатиона и сопровождалась ин- тенсивным накоплением продуктов перекисного окисления липидов.

The aim of this work is to study the biochemical organization and response of defense system in digestive gland and gills of marine bivalve Scapharca broughtoni during anoxia/ hypoxia and recovery. The results obtained show that anoxia exposure caused decreasing of total antioxidant activity, level of glutathione and intensive increasing of lipid peroxidation product.

аноксияреоксигенацияантиоксидантная системаинтегральная антирадикальная активностьокислительный стрессглутатионМДАanoxiaaerobic recoveryantioxidant systemtotal oxyradical scavenging capacityoxidative stressglutathionelipid peroxidationReferencesAbele-Oeschger D., Oeschger R. Hypoxia-induced autoxidation of haemoglobin of the benthic invertebrates Arenicola marina (Polychaeta) and Astarte borealis (Bivalvia) and the possible effects of sulfide // J. Exp. Mar. Biol. Ecol. - 1995. - V. 187. - P. 63-80.Abele-Oeschger D., Oeschger R. Hypoxia-induced autoxidation of haemoglobin of the benthic invertebrates Arenicola marina (Polychaeta) and Astarte borealis (Bivalvia) and the possible effects of sulfide // J. Exp. Mar. Biol. Ecol. - 1995. - V. 187. - P. 63-80.Bebianno M.J., Company R., Serafim A., Camus L., Cosson R.P., Fiala-Medoni A. Antioxidant systems and lipid peroxidation in Bathymodiolus azoricus from Mid-Atlantic Ridge hydrothermal vent fields // Aquat. Toxicol. - 2005. - V. 75. - P. 354-373.Bebianno M.J., Company R., Serafim A., Camus L., Cosson R.P., Fiala-Medoni A. Antioxidant systems and lipid peroxidation in Bathymodiolus azoricus from Mid-Atlantic Ridge hydrothermal vent fields // Aquat. Toxicol. - 2005. - V. 75. - P. 354-373.Bocchetti R., Fattorini D., Gambi M. C., Regoli F. Trace Metal Concentrations and Susceptibility to Oxidative Stress in the Polychaete Sabella spallanzanii (Gmelin) (Sabellidae): Potential Role of Antioxidants in Revealing Stressful Environmental Conditions in the Mediterranean // Arch. Environ. Contam. Toxicol. - 2004. - V.46. - P. 353-361.Bocchetti R., Fattorini D., Gambi M. C., Regoli F. Trace Metal Concentrations and Susceptibility to Oxidative Stress in the Polychaete Sabella spallanzanii (Gmelin) (Sabellidae): Potential Role of Antioxidants in Revealing Stressful Environmental Conditions in the Mediterranean // Arch. Environ. Contam. Toxicol. - 2004. - V.46. - P. 353-361.Buege J.A., Aust S.D. Microsomal lipid peroxidation. Methods in Enzymology, Eds. by Fleischer S., Packer L., N.Y.: Academic Press. - 1978. - P. 302-310.Buege J.A., Aust S.D. Microsomal lipid peroxidation. Methods in Enzymology, Eds. by Fleischer S., Packer L., N.Y.: Academic Press. - 1978. - P. 302-310.de Zwaan A., Cortesi P., van den Thillart G., Roos J., Storey K.B. Differential sensitivities to hypoxia by two anoxia-tolerant marine molluscs: a biochemical analysis // Mar. Biol. - 1991. - V. 111. - P. 343-351.de Zwaan A., Cortesi P., van den Thillart G., Roos J., Storey K.B. Differential sensitivities to hypoxia by two anoxia-tolerant marine molluscs: a biochemical analysis // Mar. Biol. - 1991. - V. 111. - P. 343-351.de Zwaan A., Isani G., Cottani O., Cortesi P. Long-term metabolism of erythrocytes of the arcid clam Scapharca inaequivalvis // J. Exp. Mar. Biol. Ecol. - 1995. - V. 187. - P. 27-37.de Zwaan A., Isani G., Cottani O., Cortesi P. Long-term metabolism of erythrocytes of the arcid clam Scapharca inaequivalvis // J. Exp. Mar. Biol. Ecol. - 1995. - V. 187. - P. 27-37.de Zwaan A., Eertman R.H.M. Anoxic or air survival of bivalves and other euroxic invertebrates as useful response to environmental stress - a comprehensive review // Comp. Biochem. Physiol. - 1996. - V. 113 (C). - P. 299-312.de Zwaan A., Eertman R.H.M. Anoxic or air survival of bivalves and other euroxic invertebrates as useful response to environmental stress - a comprehensive review // Comp. Biochem. Physiol. - 1996. - V. 113 (C). - P. 299-312.Dovzhenko N.V., Kurilenko A.V., Belcheva N.N., Chelomin V.P. Cadmium-induced oxidative stress in the bivalve mollusk Modiolus modiolus // Russian J. Mar. Biol. - 2005. - V. 31. - P. 309-313.Dovzhenko N.V., Kurilenko A.V., Belcheva N.N., Chelomin V.P. Cadmium-induced oxidative stress in the bivalve mollusk Modiolus modiolus // Russian J. Mar. Biol. - 2005. - V. 31. - P. 309-313.Eertman R.H.M., Wagenvoort A.J., Hummel H. and Smaal A.C. "Survival in air" of the blue mussel Mytilus edulis L. as a sensitive response to pollution-induced environmental stress // J. Exp. Mar. Biol. Ecol. - 1993. - V. 170. - P. 179-195.Eertman R.H.M., Wagenvoort A.J., Hummel H. and Smaal A.C. "Survival in air" of the blue mussel Mytilus edulis L. as a sensitive response to pollution-induced environmental stress // J. Exp. Mar. Biol. Ecol. - 1993. - V. 170. - P. 179-195.Eertman R.H.M., de Zwaan A. Survival of the fittest: resistance of mussels to aerial exposure. In: Biomonitoring of Coastal Waters and Estuaries. Eds. by Kramer K.J.M // CRC Press, Boca Raton. - 1994. - P. 269-284.Eertman R.H.M., de Zwaan A. Survival of the fittest: resistance of mussels to aerial exposure. In: Biomonitoring of Coastal Waters and Estuaries. Eds. by Kramer K.J.M // CRC Press, Boca Raton. - 1994. - P. 269-284.Greenberg C.S., Gaddock P.R. Rapid single-step membrane protein assay. Clin. Chem. - 1982. - V. 28 (7). - P. 1725-1726.Greenberg C.S., Gaddock P.R. Rapid single-step membrane protein assay. Clin. Chem. - 1982. - V. 28 (7). - P. 1725-1726.Hassoun E.A., Stohs S.J. Cadmium-induced production of superoxide anion and nitric oxide, DNA single strand breaks and lactate dehydrogenase leakage in J774A. 1 cell cultures // Toxicology. - 1996. -V. 112 (2-3). - P. 219-226.Hassoun E.A., Stohs S.J. Cadmium-induced production of superoxide anion and nitric oxide, DNA single strand breaks and lactate dehydrogenase leakage in J774A. 1 cell cultures // Toxicology. - 1996. -V. 112 (2-3). - P. 219-226.Misra H.P., Fridovich I. The generation of superoxide radical during the autoxidation of haemoglobin // J. Biol. Chem. - 1972. - V. 247. - P. 6960-6962.Misra H.P., Fridovich I. The generation of superoxide radical during the autoxidation of haemoglobin // J. Biol. Chem. - 1972. - V. 247. - P. 6960-6962.Moron M.S., Depierre J.W., Mannervik B. Levels of glutathione, glutathione reductase and glutathione stransferase activities in rat lung and liver. Biochim. Biophys. Acta. - 1979. - V.582. - P. 67-78.Moron M.S., Depierre J.W., Mannervik B. Levels of glutathione, glutathione reductase and glutathione stransferase activities in rat lung and liver. Biochim. Biophys. Acta. - 1979. - V.582. - P. 67-78.Regoli F., Winston G.W., Mastrangelo V. et al. Total oxyradical scavenging capacity in mussel Mytilus sp. as a new index of biological resistance to oxidative stress. Chemosphere. - 1998. - V. 37. - P. 2773-2783.Regoli F., Winston G.W., Mastrangelo V. et al. Total oxyradical scavenging capacity in mussel Mytilus sp. as a new index of biological resistance to oxidative stress. Chemosphere. - 1998. - V. 37. - P. 2773-2783.Regoli F., Nigro M., Bompadre S. Winston G.W. Total oxidant scavenging capacity (TOS C) of microsomal and cytosolic fraction from Antarctic, Arctic and Mediterranean scallops: differentiation between three potent oxidants // Aquat. Toxicol. - 2000. - V. 49. - P. 13-25.Regoli F., Nigro M., Bompadre S. Winston G.W. Total oxidant scavenging capacity (TOS C) of microsomal and cytosolic fraction from Antarctic, Arctic and Mediterranean scallops: differentiation between three potent oxidants // Aquat. Toxicol. - 2000. - V. 49. - P. 13-25.Regoli F. Total oxyradical scavenging capacity (TOS C) in polluted and translocated mussels: a predictive biomarker of oxidative stress // Aquatic Toxicol. - 2000. - V. 50. - P. 351-361.Regoli F. Total oxyradical scavenging capacity (TOS C) in polluted and translocated mussels: a predictive biomarker of oxidative stress // Aquatic Toxicol. - 2000. - V. 50. - P. 351-361.Regoli F., Cerrano C., Chierici E., Bompadre S., Bavestrello G. Susceptibility to oxidative stress of the Mediterranean demosponge Petrosia ficiformis: role of endosymbionts and solar irradiance // Mar. Biol. - 2000. - V. 137. - P. 453-461.Regoli F., Cerrano C., Chierici E., Bompadre S., Bavestrello G. Susceptibility to oxidative stress of the Mediterranean demosponge Petrosia ficiformis: role of endosymbionts and solar irradiance // Mar. Biol. - 2000. - V. 137. - P. 453-461.Regoli F., Nigro M., Chierici E., Cerrano C., Schiapparelli S., Totti C., Bavestrello G. Variations of antioxidant efficiency and presence of endosymbiotic diatoms in the Antarctic porifera Haliclona dancoi // Mar. Environ. Res. - 2004. - V. 58. - P. 637-640.Regoli F., Nigro M., Chierici E., Cerrano C., Schiapparelli S., Totti C., Bavestrello G. Variations of antioxidant efficiency and presence of endosymbiotic diatoms in the Antarctic porifera Haliclona dancoi // Mar. Environ. Res. - 2004. - V. 58. - P. 637-640.Regoli F., Gorbi S., Fattorini D., Tedesco S., Notti A., Machella N., Bocchetti R., Benedetti M., Piva F. Use of the Land Snail Helix aspersa as Sentinel Organism for Monitoring Ecotoxicologic Effects of Urban Pollution: An Integrated Approach // Environ. Health Perspect. - 2006. - V. 114. - P. 63-69.Regoli F., Gorbi S., Fattorini D., Tedesco S., Notti A., Machella N., Bocchetti R., Benedetti M., Piva F. Use of the Land Snail Helix aspersa as Sentinel Organism for Monitoring Ecotoxicologic Effects of Urban Pollution: An Integrated Approach // Environ. Health Perspect. - 2006. - V. 114. - P. 63-69.Sandrini J. Z., Regoli F., Fattorini D., Notti A., Inacio A.F., Linde-Arias A.R., Laurino J., Bainy A.C.D., Marins L.F.F., Monserrat J.M. Short-term responses to cadmium exposure in the estuarine polychaete Laeonereis acuta (Polychaeta, Nereididae): subcellular distribution and oxidative stress generation // Environ. Toxicol. Chem. - 2006. - V. 25. - P. 1337-1344.Sandrini J. Z., Regoli F., Fattorini D., Notti A., Inacio A.F., Linde-Arias A.R., Laurino J., Bainy A.C.D., Marins L.F.F., Monserrat J.M. Short-term responses to cadmium exposure in the estuarine polychaete Laeonereis acuta (Polychaeta, Nereididae): subcellular distribution and oxidative stress generation // Environ. Toxicol. Chem. - 2006. - V. 25. - P. 1337-1344.Weber R.E., Lykke-Madsen M., Bang A., de Zwaan A., Cortesi P. Effects of cadmium on anoxic survival, haematology, erythrocytic volume regulation and haemoglobin-oxygen affinity in the marine bivalve Scapharca inaequivalvis // J. Exp. Mar. Biol. Ecol. - 1990. - V. 144. - P. 29-38.Weber R.E., Lykke-Madsen M., Bang A., de Zwaan A., Cortesi P. Effects of cadmium on anoxic survival, haematology, erythrocytic volume regulation and haemoglobin-oxygen affinity in the marine bivalve Scapharca inaequivalvis // J. Exp. Mar. Biol. Ecol. - 1990. - V. 144. - P. 29-38.

The authors declare that there are no conflicts of interest present.