geomgouГеографическая среда и живые системыGeographical Environment and Living Systems2712-76132712-7621Московский государственный областной университетgeomgou-771Research ArticleСтатьиИДЕНТИФИКАЦИЯ И ЧАСТИЧНАЯ ХАРАКТЕРИСТИКА ДВУХ ВЫСОКОМОЛЕКУЛЯРНЫХ КАДМИЙ-СВЯЗЫВАЮЩИХ БЕЛКОВ ПРИМОРСКОГО ГРЕБЕШКА MIZUHOPECTEN YESSOENSISINVOLVMENT OF HIGH MOLECULAR WEIGHT Cd -BINDING PROTEINS IN Cd-ACCUMULATION IN THE SCALLOP MIZUHOPECTEN YESSOENSISЖуковскаяАвианна Фаязовнаavianna@poi.dvo.ruБельчеваНина Николаевнаbelcheva@poi.dvo.ruЧеломинВиктор Павловичchelomin@poi.dvo.ruТихоокеанский Океанологический Институт им. В. И. Ильичева Дальневосточного отделения РАН (Владивосток)Russian Federation201127052022033642Copyright © Жуковская А.Ф., Бельчева Н.Н., Челомин В.П., 20222022Жуковская А.Ф., Бельчева Н.Н., Челомин В.П.Жуковская А.Ф., Бельчева Н.Н., Челомин В.П.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.geoecosreda.ru/jour/article/view/771

Многое уже известно о кадмий-связы- вающих лигандах в других видах семейства Pectinidae, однако ничего не известно о дальневосточном пред- ставителе этого семейства - приморском гребешке Mizuhopecten yessoensis. В работе были исследова- ны кадмий-связывающие лиганды в пищеваритель- ной железе M. yessoensis. В данной работе впервые обнаружены в клетках пищеварительной железы Mizuhopecten yessoensis термостабильные, устойчи- вые к органическим растворителям высокомолеку- лярные кадмий-связывающие лиганды. Уникальными особенностями Cd-связывающих белков данного вида является не только вес (72 кДа и 43 кДа), но также и спектроскопические характеристики (ƒ 254/ ƒ 280 нм > 2); 10 SH - групп на два белка. Результаты исследо- ваний накопления кадмия в пищеварительной железе приморского гребешка и исследование распределения кадмия среди цитоплазматических белков в особях из мест с разной степенью антропогенного вмешательс- тва свидетельствуют о хорошо развитых механизмах адаптации у Mizuhopecten yessoensis к кадмию.

Mollusks have long been known to naturally accumulate metals to high concentrations, particularly in the digestive gland. Among them scallops have able to accumulate toxic Cd to concentrations far exceeding those in the surrounding seawater. We have studied the cadmium-binding ligands in the digestive gland of M. yessoensis The results have revealed two high molecular weight proteins with a 72 and 43 kDa in M. yessoensis with specifi c spectroscopic relation (ƒ 254/ ƒ 280 нм > 2) and 10 SH-groups for both proteins. The results of the study of cadmium accumulation in the digestive gland of the scallop and the distribution of cadmium among cytoplasmic proteins in the M. yessoensis from locations with varying degrees of anthropogenic impact showed a well-developed mechanism of adaptation in M. yessoensis to highly toxic cadmium.

кадмий-связывающие белкиМТ-подобные белкитяжелые металлыприморский гребешокадаптацияCd-binding proteinsMT-like proteinsheavy metalsscallopBivalviabiochemical adaptationReferencesНиканоров А.М., Жулидов А.В., Покаржевский А.Д. Биомониторинг тяжелых металлов в пре- сных экосистемах. Л.: Гидрометеоиздат, 1985.Никаноров А.М., Жулидов А.В., Покаржевский А.Д. Биомониторинг тяжелых металлов в пре- сных экосистемах. Л.: Гидрометеоиздат, 1985.Челомин В.П. Экотоксикологические аспекты биоаккумуляции кадмия (на примере морских двустворчатых моллюсков). Автореф. дис.  док. биол. наук. Владивосток: ТОЙ ДВО РАН. 1998.Челомин В.П. Экотоксикологические аспекты биоаккумуляции кадмия (на примере морских двустворчатых моллюсков). Автореф. дис.  док. биол. наук. Владивосток: ТОЙ ДВО РАН. 1998.Bebiano M., Serafi m M. and Rita M. Involvement of metallothionein in cadmium accumulation and elimination in the clam Ruditapes decussata // Bull. Environ. Contam. Toxicol. 1994. V. 53.Bebiano M., Serafi m M. and Rita M. Involvement of metallothionein in cadmium accumulation and elimination in the clam Ruditapes decussata // Bull. Environ. Contam. Toxicol. 1994. V. 53.Bustamante P., Miramand P. Interspecifi c and geographical variations of trace element concentrations in Pectinidae from European waters // Chemosphere. 2004. V. 57.Bustamante P., Miramand P. Interspecifi c and geographical variations of trace element concentrations in Pectinidae from European waters // Chemosphere. 2004. V. 57.Dallinger R., Berger B., Gruber C., Stьrzenbaum S. Metallothioneins in Terrestrial Invertebrates: Structural Aspects, Biological Signifi cance, and Implications for their Use as Biomarkers // Cell. Mol. Biology. 2000. V. 46(2).Dallinger R., Berger B., Gruber C., Stьrzenbaum S. Metallothioneins in Terrestrial Invertebrates: Structural Aspects, Biological Signifi cance, and Implications for their Use as Biomarkers // Cell. Mol. Biology. 2000. V. 46(2).Dallinger R., Berger B., Hunziker P.E., Birchler N., Hauer C.R. and Jeremias H.R. Kagi. Purifi cation and primary structure of snail metallothionein. Similarity of the N-terminal sequence with histones H4 and H2A // Eur. J. Biochem. 1993. V. 216.Dallinger R., Berger B., Hunziker P.E., Birchler N., Hauer C.R. and Jeremias H.R. Kagi. Purifi cation and primary structure of snail metallothionein. Similarity of the N-terminal sequence with histones H4 and H2A // Eur. J. Biochem. 1993. V. 216.Giguere A., Coulliard Y., Campbell P. G.C., et al. Steady-state distribution of metals among metallothionein and other cytosolic ligands and links to cytotoxicity in bivalves living along a polymetallic gradient // Aquatic Toxicology. 2003. V. 64.Giguere A., Coulliard Y., Campbell P. G.C., et al. Steady-state distribution of metals among metallothionein and other cytosolic ligands and links to cytotoxicity in bivalves living along a polymetallic gradient // Aquatic Toxicology. 2003. V. 64.Hamer D.H. Metallothionein // Ann. Rev. Biochem. 1986. V. 55.Hamer D.H. Metallothionein // Ann. Rev. Biochem. 1986. V. 55.Jin-Sung Park, Soohee Chung, Il-Seon Park, Yangsun Kim, Chul -Hwan Koh, In-Sook Lee. Purifi cation and characterization of metallothionein-like cadmium-binding protein from Asian perwinkle Littorina brevicula // Comparative Biochemistry and Physiology Part C. 2002. V. 131.Jin-Sung Park, Soohee Chung, Il-Seon Park, Yangsun Kim, Chul -Hwan Koh, In-Sook Lee. Purifi cation and characterization of metallothionein-like cadmium-binding protein from Asian perwinkle Littorina brevicula // Comparative Biochemistry and Physiology Part C. 2002. V. 131.Julshamn K., Andersen K.-J. Subcellular distribution of major and minor elements in unexposed mollusks in western Norway-I. Th e distribution and binding of cadmium, zinc and copper in the liver and the digestive system of the oyster Ostrea edulis // Comp. Biochem. Physiol. 1983. V. 75A.Julshamn K., Andersen K.-J. Subcellular distribution of major and minor elements in unexposed mollusks in western Norway-I. Th e distribution and binding of cadmium, zinc and copper in the liver and the digestive system of the oyster Ostrea edulis // Comp. Biochem. Physiol. 1983. V. 75A.Kagi J.H.R., Kojima Y. Chemistry and biochemistry of metallothionein. Metallothionein II. Eds. by Kagi J.H.R., Kojima Y., Basel: Birkhauser Verlag. 1987.Kagi J.H.R., Kojima Y. Chemistry and biochemistry of metallothionein. Metallothionein II. Eds. by Kagi J.H.R., Kojima Y., Basel: Birkhauser Verlag. 1987.Laemly U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. // Nature. 1970. № 5259.Laemly U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. // Nature. 1970. № 5259.Lowry O. H., Rosebrough N. J., Farr, A. L., Randall R. J. Protein measurement with the Folin phenol reagent // Journal of Biological Chemistry. 1951. V. 193.Lowry O. H., Rosebrough N. J., Farr, A. L., Randall R. J. Protein measurement with the Folin phenol reagent // Journal of Biological Chemistry. 1951. V. 193.Metian Marc, Warnau Michel, Bustamante Paco, et. al. Interspecifi c comparisons of Cd bioaccumulation in European Pectinidae (Chlamys varia and Pecten maximus) // Journal of Experimental Marine Biology and Ecology. 2007. V. 353.Metian Marc, Warnau Michel, Bustamante Paco, et. al. Interspecifi c comparisons of Cd bioaccumulation in European Pectinidae (Chlamys varia and Pecten maximus) // Journal of Experimental Marine Biology and Ecology. 2007. V. 353.Nolan C.V., Duke E.J. Cadmium accumulation and toxicity in Mytilus edulis: involvement of metallothioneins and heavy-molecular weight protein // Aquat. Toxicol. 1983. V. 4.Nolan C.V., Duke E.J. Cadmium accumulation and toxicity in Mytilus edulis: involvement of metallothioneins and heavy-molecular weight protein // Aquat. Toxicol. 1983. V. 4.Petering D.H., Fowler B.A. Discussion summary. Role of metallothionein and related proteins in metal metabolism and toxicity: problems and perspectives // Environ. Health Perspect. 1986. V. 65.Petering D.H., Fowler B.A. Discussion summary. Role of metallothionein and related proteins in metal metabolism and toxicity: problems and perspectives // Environ. Health Perspect. 1986. V. 65.Ponzano E., Dondero E., Bouquegneau J.-M., Sack R., Hunziker P., Viarengo A. Purifi cation and biochemical characterization of cadmium metallothionein from the digestive gland of the Antarctic scallop Adamussium colbecki (Smith, 1902) // Polar Biology. 2001. V. 24.Ponzano E., Dondero E., Bouquegneau J.-M., Sack R., Hunziker P., Viarengo A. Purifi cation and biochemical characterization of cadmium metallothionein from the digestive gland of the Antarctic scallop Adamussium colbecki (Smith, 1902) // Polar Biology. 2001. V. 24.Roesijadi G. Metallothioneins in metal regulation and toxicity in aquatic animals (Review) // Aquat. Toxicol. 1992. V. 22.Roesijadi G. Metallothioneins in metal regulation and toxicity in aquatic animals (Review) // Aquat. Toxicol. 1992. V. 22.Roesijadi G., Fowler B. A. Purifi cation of invertebrate metallothioneins // Methods in Enzymology. - 1991. V. 205.Roesijadi G., Fowler B. A. Purifi cation of invertebrate metallothioneins // Methods in Enzymology. - 1991. V. 205.Stone H.C., Wilson S.B., Overnell J. Cd-binding proteins in the scallop Pecten maximus // Environ. Health Perspect. 1986. V. 65.Stone H.C., Wilson S.B., Overnell J. Cd-binding proteins in the scallop Pecten maximus // Environ. Health Perspect. 1986. V. 65.Viarengo A., Canesi L., Massu-Cotelli A., Ponzano E., Orunesu M. Cu, Zn, Cd content in diff erent tissues of the Antarctic scallop Adamussium colbecki (Smith 1902): role of metallothionein in the homeostasis and in the detoxifi cation of heavy metals // Mar. Env. Res. - 1993. - V. 35. - P. 216-217.Viarengo A., Canesi L., Massu-Cotelli A., Ponzano E., Orunesu M. Cu, Zn, Cd content in diff erent tissues of the Antarctic scallop Adamussium colbecki (Smith 1902): role of metallothionein in the homeostasis and in the detoxifi cation of heavy metals // Mar. Env. Res. - 1993. - V. 35. - P. 216-217.

The authors declare that there are no conflicts of interest present.