geomgouГеографическая среда и живые системыGeographical Environment and Living Systems2712-76132712-7621Московский государственный областной университетgeomgou-495Research ArticleБИОЛОГИЯАКТИВАЦИЯ tlr9-, NF-kB-, JNK/p38И IRF-СИГНАЛЬНЫХ ПУТЕЙ В МЕЗЕНХИМАЛЬНЫХ СТВОЛОВЫХ КЛЕТКАХ ЧЕЛОВЕКАACTIVATION OF TLR9-, NF-KB-, JNK/P38И IRF-SIGNAL PATHWAYS IN MESENCHYMAL STEM CELLSКостюкСветлана ВикторовнаKostuykS. .oksana.chvartack@mail.ruЧвартацкаяОксана ВикторовнаChvartatskayaO. .oksana.chvartack@mail.ruСевастьяноваГалина АндреевнаSevastyanovaG. .oksana.chvartack@mail.ruВейкоНаталья НиколаевнаVeikoN. .oksana.chvartack@mail.ruМедико-генетический научный центр РАМНРоссияResearch Centre for Medical Genetics under the Russian Academy of Medical SciencesRussian FederationМосковский педагогический государственный университетРоссияMoscow State Pedagogical UniversityRussian Federation201306052022023844Copyright © Костюк С.В., Чвартацкая О.В., Севастьянова Г.А., Вейко Н.Н., 20222022Костюк С.В., Чвартацкая О.В., Севастьянова Г.А., Вейко Н.Н.Kostuyk S..., Chvartatskaya O..., Sevastyanova G..., Veiko N...Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.geoecosreda.ru/jour/article/view/495

Известно, что ГЦ-обогащенные фрагменты внеклеточной ДНК (ГЦ-вкДНК) накапливаются в плазме крови при патологии, и ранее нами было показано, что ГЦ-вкДНК активирует TLR9-зависимый сигнальный путь, увеличивая количество мРНК и белка TLR9 в клетках. В данной работе была смоделирована in vitro ситуация, когда эндогенные мезенхимальные стволовые клетки (МСК), мигрирующие из ниши в очаги повреждения, или МСК, введенные в организм с целью терапии, «встречаются» с ГЦ-вкДНК, которая содержит сайты - лиганды TLR9. Было показано, что плазмида (п(рДНК)), содержащая в своем составе фрагменты ГЦ-вкДНК, активирует в МСК TLR9-сигнальный путь и связанные с ним NF-kB-, JNK/p38-, IRF-сигнальные пути.

It is well known that GC-rich cell free DNA (GC-cfDNA) accumulates in the blood plasma at disease. We have previously shown that GC-cfDNA activates TLR9-dependent signal pathway, thereby increasing TLR9 and its signal pathway adapter MyD88 expression. We study in details the influence of the GC-DNA content on the activation of genes related to TLR9-dependent signal pathways. We have shown that GC-DNA increases the expression of TLR signal pathway and TLR9-dependent signal pathways such as NF-kB- , JNK/p38-and IRF-signal pathways. Thus, MSCs respond to changes in sfDNA GC-content by activating TLR-, NF-kB-, JNK/p38- and IRF-signal pathways.

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The authors declare that there are no conflicts of interest present.