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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">geomgou</journal-id><journal-title-group><journal-title xml:lang="ru">Географическая среда и живые системы</journal-title><trans-title-group xml:lang="en"><trans-title>Geographical Environment and Living Systems</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2712-7613</issn><issn pub-type="epub">2712-7621</issn><publisher><publisher-name>Московский государственный областной университет</publisher-name></publisher></journal-meta><article-meta><article-id custom-type="elpub" pub-id-type="custom">geomgou-468</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>БИОЛОГИЯ</subject></subj-group></article-categories><title-group><article-title>ВЫДЕЛЕНИЕ КУЛЬТУРЫ (ACHROMOBACTER SP.) ДЕГРАДАТОРА ПАРАФИНА ИЗ ПОЧВЫ</article-title><trans-title-group xml:lang="en"><trans-title>ISOLATION OF PARAFFIN-DEGRADER ACHROMOBACTER SP. FROM AZARSHAR SOILS (EAST AZARBAYJAN, IRAN)</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Мобайен</surname><given-names>Надех</given-names></name><name name-style="western" xml:lang="en"><surname>Mobaiyen</surname><given-names>H. .</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Садигдбайан</surname><given-names>Хосров</given-names></name><name name-style="western" xml:lang="en"><surname>Sadigbayan</surname><given-names>K. .</given-names></name></name-alternatives><email xlink:type="simple">azmbi@mail.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Насери</surname><given-names>Абдул-Гусейн</given-names></name><name name-style="western" xml:lang="en"><surname>Naseri</surname><given-names>A. -H.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Дехнад</surname><given-names>Алиреза</given-names></name><name name-style="western" xml:lang="en"><surname>Dehnad</surname><given-names>A. .</given-names></name></name-alternatives><email xlink:type="simple">azmbi@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Модир-Шахла</surname><given-names>Насер</given-names></name><name name-style="western" xml:lang="en"><surname>Modirshahla</surname><given-names>N. .</given-names></name></name-alternatives><email xlink:type="simple">azmbi@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ахмадпор</surname><given-names>Фархад</given-names></name><name name-style="western" xml:lang="en"><surname>Ahmadpor</surname><given-names>F. .</given-names></name></name-alternatives><email xlink:type="simple">azmbi@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Исламский свободный университет</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Islamic Azad University</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Институт микробиологии НАН Азербайджана</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Institute of Microbiology, National Academy of Sciences of Azerbaijan</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>Тебризский университет</institution><country>Россия</country></aff><aff xml:lang="en"><institution>University of Tabriz</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2014</year></pub-date><pub-date pub-type="epub"><day>06</day><month>05</month><year>2022</year></pub-date><volume>0</volume><issue>2</issue><fpage>52</fpage><lpage>57</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Мобайен Н..., Садигдбайан Х..., Насери А.-., Дехнад А..., Модир-Шахла Н..., Ахмадпор Ф..., 2022</copyright-statement><copyright-year>2022</copyright-year><copyright-holder xml:lang="ru">Мобайен Н., Садигдбайан Х., Насери А., Дехнад А., Модир-Шахла Н., Ахмадпор Ф.</copyright-holder><copyright-holder xml:lang="en">Mobaiyen H..., Sadigbayan K..., Naseri A.-., Dehnad A..., Modirshahla N..., Ahmadpor F...</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.geoecosreda.ru/jour/article/view/468">https://www.geoecosreda.ru/jour/article/view/468</self-uri><abstract><p>Из аридных почв (Азершехр, Восточный Азербайджан, Иран) были изолированы и получены 10 чистых культур микроорганизмов. Из всех выделенных культур только штаммы F10 и E12, идентифицированные с помощью молекулярных тестов и полимеразной цепной реакции (PCR, ПЦР) как Achromobacter sp., были способны разлагать жидкий парафин более чем на 90%. Основной промежуточный продукт разложения парафина был идентифицирован как уксусная кислота. Анализ микрофлоры различных почв показал возможность получения штаммов бактерий, которые в свою очередь могут быть использованы для получения с помощью методов генетической модификации микробных препаратов - перспективных для деградации органических соединений (подобно парафинам).</p></abstract><trans-abstract xml:lang="en"><p>Ten pure colonies of microorganisms were isolated and purified from arid soils of Azarshahr (East Azerbaijan, Iran). Of all the isolates, and only strains F10 and E12, identified by molecular tests and polymerase chain reaction (PCR) as Achromobacter sp., could degrade liquid paraffin by more than 90%. The primary intermediate product of decomposed paraffin was identified as acetic acid. Analysis of various soil microflora showed the possibility of obtaining strains of bacteria, which in turn can be used to produce microbial preparations, promising for the degradation of organic compounds (like paraffin), by the methods of genetic modification.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>жидкие парафины</kwd><kwd>штаммы Achromobacter sp</kwd><kwd>полимеразная цепная реакция (PCR)</kwd><kwd>biodegradation</kwd><kwd>Liquid paraffin</kwd><kwd>Achromobacter</kwd><kwd>polymerase chain reaction</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Alexander M. Introduction to soil microbiology. - NY and L.: John Wiley and Sons, Inc., 1998. - 233 р.</mixed-citation><mixed-citation xml:lang="en">Alexander M. Introduction to soil microbiology. - NY and L.: John Wiley and Sons, Inc., 1998. - 233 р.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Banwar L.A.L. Prevention of paraffin deposition in oil well Thermophilic paraffin degrading strain Geobacillus kaustophilus / Banwar L.A.L. et al. -New Delhi, India: Petrotech. - 2009. - 224 р.</mixed-citation><mixed-citation xml:lang="en">Banwar L.A.L. Prevention of paraffin deposition in oil well Thermophilic paraffin degrading strain Geobacillus kaustophilus / Banwar L.A.L. et al. -New Delhi, India: Petrotech. - 2009. - 224 р.</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Bardi L. Hydrocarbon degradation by a soil microbial population with β - cyclodextrin as surfactant to enhance bioavailability / Bardi L. et al. // J. Enzyme and Microbial Technology. - 2000. - V. 27 (Iss. 9). - P. 709-713.</mixed-citation><mixed-citation xml:lang="en">Bardi L. Hydrocarbon degradation by a soil microbial population with β - cyclodextrin as surfactant to enhance bioavailability / Bardi L. et al. // J. Enzyme and Microbial Technology. - 2000. - V. 27 (Iss. 9). - P. 709-713.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Chorom M. Bioremediation of a crude oil-Polluted soil by application of fertilizers / Chorom M. et al. // Iran. - J. Environ. Health. Sci. Eng. - 2010. - V. 7 (№ 4). - P. 324-326.</mixed-citation><mixed-citation xml:lang="en">Chorom M. Bioremediation of a crude oil-Polluted soil by application of fertilizers / Chorom M. et al. // Iran. - J. Environ. Health. Sci. Eng. - 2010. - V. 7 (№ 4). - P. 324-326.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Garcia-Rivero M. Organic solvents improve hydrocarbon desorption and biodegradation in highly contaminated weathered soils / Garcia-Rivero M. et al. // Environ. Eng. Sci. - 2007. - № 6. - Р. 389-395.</mixed-citation><mixed-citation xml:lang="en">Garcia-Rivero M. Organic solvents improve hydrocarbon desorption and biodegradation in highly contaminated weathered soils / Garcia-Rivero M. et al. // Environ. Eng. Sci. - 2007. - № 6. - Р. 389-395.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Guo Y. A multilocus phylogeny of the Streptomyces griseus 16S rRNA gene clade: use of multilocus sequence analysis for streptomycete systematic / Y. Guo et al. // International Journal of Systematic and Evolutionary Microbiology. - 2008. - V. 58. - P. 149-159.</mixed-citation><mixed-citation xml:lang="en">Guo Y. A multilocus phylogeny of the Streptomyces griseus 16S rRNA gene clade: use of multilocus sequence analysis for streptomycete systematic / Y. Guo et al. // International Journal of Systematic and Evolutionary Microbiology. - 2008. - V. 58. - P. 149-159.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Jussara P., Francisca P. Biodegradation of crude oil in sandy sediment // Jour. International Biodeterioration &amp; Biodegradation. - 1999. - V. 44. - P. 87-92.</mixed-citation><mixed-citation xml:lang="en">Jussara P., Francisca P. Biodegradation of crude oil in sandy sediment // Jour. International Biodeterioration &amp; Biodegradation. - 1999. - V. 44. - P. 87-92.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Kaplan C.W., Kitts C.L. Bacterial succession in a petroleum land treatment unit // Applied an Environmental Microbiology. - 2004. - V. 70 (№ 3). -Р. 1777-1786.</mixed-citation><mixed-citation xml:lang="en">Kaplan C.W., Kitts C.L. Bacterial succession in a petroleum land treatment unit // Applied an Environmental Microbiology. - 2004. - V. 70 (№ 3). -Р. 1777-1786.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Naggar A.Y. Bioremediation of Paraffinic and Poly nuclear aromatic hydrocarbons Using Laser irradiated Bacillus amyloliquefaciens / Naggar A.Y. et al. // Journal of American Science. - 2010. - V.6 (№ 10). - P. 661-670.</mixed-citation><mixed-citation xml:lang="en">Naggar A.Y. Bioremediation of Paraffinic and Poly nuclear aromatic hydrocarbons Using Laser irradiated Bacillus amyloliquefaciens / Naggar A.Y. et al. // Journal of American Science. - 2010. - V.6 (№ 10). - P. 661-670.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Olivieri R. Microbial degradation of oil spills enhanced by a slow release fertilizer / Olivieri R. et al. // Applied and Environmental Microbiology. - 1976. - V. 31 (5). -P. 629-634.</mixed-citation><mixed-citation xml:lang="en">Olivieri R. Microbial degradation of oil spills enhanced by a slow release fertilizer / Olivieri R. et al. // Applied and Environmental Microbiology. - 1976. - V. 31 (5). -P. 629-634.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Sadeghazad A., Ghaemi N. Microbial prevention of wax precipitation in crude oil by biodegradation mechanism // SPE Asia Pacific Oil and Gas Conference and Exibition (Paper SPE 80529). - Jakarta, Indonesia: Society of Petroleum Engineers, 2003. - Р. 1-11.</mixed-citation><mixed-citation xml:lang="en">Sadeghazad A., Ghaemi N. Microbial prevention of wax precipitation in crude oil by biodegradation mechanism // SPE Asia Pacific Oil and Gas Conference and Exibition (Paper SPE 80529). - Jakarta, Indonesia: Society of Petroleum Engineers, 2003. - Р. 1-11.</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Southam G. Structural characterization of the hydrocarbon degrading bacteria-oil interface: implications for bioremediation // Jour. International Biodeterioration &amp; Biodegradation. - 2001. - V. 47 (Iss. 4). - P. 197-201.</mixed-citation><mixed-citation xml:lang="en">Southam G. Structural characterization of the hydrocarbon degrading bacteria-oil interface: implications for bioremediation // Jour. International Biodeterioration &amp; Biodegradation. - 2001. - V. 47 (Iss. 4). - P. 197-201.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
