geomgouГеографическая среда и живые системыGeographical Environment and Living Systems2712-76132712-7621Московский государственный областной университетgeomgou-433Research ArticleРАЗДЕЛ I. БИОЛОГИЧЕСКИЕ НАУКИSECTION I. BIOLOGICAL SCIENCESИЗУЧЕНИЕ АКТИВНОСТИ ФЕНИЛАЛАНИН-АММИАК-ЛИАЗЫ, КОЛИЧЕСТВО АНТОЦИАНИНОВ И ФЛАВОНОИДОВ ПРИ РОСТЕ И СОЗРЕВАНИИ ПЛОДОВ ЯБЛОНИ НА МАТЕРИНСКОМ ДЕРЕВЕSTUDY OF PHENYLALANINE AMMONIA-LYASE ACTIVITY, ANTHOCYANIN AND FLAVONOID LEVELS AT DIFFERENT STAGES OF APPLE FRUIT DEVELOPMENT ON MOTHER TREEКулиеваСевиндж МобилQulievaazmbi@mail.ruГюльахмедовСахиб ГурбанGulahmadovS. .azmbi@mail.ruКулиевАкиф АлекберQulievA. .azmbi@mail.ruБакинский государственный университетРоссияBaku State UniversityRussian Federation201406052022052431Copyright © Кулиева С.М., Гюльахмедов С.Г., Кулиев А.А., 20222022Кулиева С.М., Гюльахмедов С.Г., Кулиев А.А.Кулиева С.М., Gulahmadov S..., Quliev A...Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.geoecosreda.ru/jour/article/view/433

Изучена динамика изменения активности фенилаланин-аммиак-лиазы (ФАЛ), а также количества антоцианинов и флавоноидов в плодах яблони двух сортов (Malus domestica Borkh.) в ходе их роста и созревания на материнском дереве. В зависимости от степени созревания и сортового состава плодов все три исследуемые показатели достоверно отличались между собою. Кривые, отражающие изменение активности ФАЛ, имели два пика. На средней стадии роста плодов активность ФАЛ значительно уменьшилась. Обнаружена положительная корреляция между уровнем активности ФАЛ и общим количеством флавоноидов. С антоцианинами такая корреляция не обнаружена.

We report the phenylalanine ammonia-lyase (PAL) activity, anthocyanin and flavonoid levels at different stages of apple fruit (Malus domestica Borkh.) development on mother trees. We have observed a wide variation in the level of the PAL activity, anthocyanin and total flavonoid levels in different apple cultivars and at different stages of development. The curves showing the change in the PAL activity have two distinct peaks. The PAL activity was found to be the highest in immature fruit, dropped to low levels during development, and then rose upon ripening. We have found a significant correlation between the average PAL activity over the all developmental stages and the final concentration of total flavonoids; however, apparent correlation between the average PAL activity and final anthocyanin concentration has not been observed.

фенилаланин-аммиак-лиаза (ФАЛ)антоцианиныфлавоноидыяблоко (Malus domestica Borkh.)phenylalanine ammonia-lyase (PAL)anthocyaninflavonoidapple fruit (Malus domestica Borkh.)maturationReferencesКулиева С.М., Гюльахмедов С.Г. Фенилаланин-аммиак-лиаза плодов яблони (Pyrus domestica Borkh.) // Труды ин-та ботаники НАНАз. - 2012. - Т. 32. - С. 206-209.Кулиева С.М., Гюльахмедов С.Г. Фенилаланин-аммиак-лиаза плодов яблони (Pyrus domestica Borkh.) // Труды ин-та ботаники НАНАз. - 2012. - Т. 32. - С. 206-209.Лаанест Л.Э., Маргна УВ. Роль фенилаланин-амиак-лиазы в накоплении флавоноидов в проростках гречихи // Физиология растений. - 1972. - Т. 19 (вып. 6). - С. 1157-1164.Лаанест Л.Э., Маргна УВ. Роль фенилаланин-амиак-лиазы в накоплении флавоноидов в проростках гречихи // Физиология растений. - 1972. - Т. 19 (вып. 6). - С. 1157-1164.Олениченко Н.А., Загоскина Н.В. Ответная реакция озимой пшеницы на действие низких температур: образование фенольных соединений и активность L-фенилаланин-амиак-лиазы // Прикл. биохим. и микробиол. - 2005. - Т. 41 (№ 6). - С. 681-685.Олениченко Н.А., Загоскина Н.В. Ответная реакция озимой пшеницы на действие низких температур: образование фенольных соединений и активность L-фенилаланин-амиак-лиазы // Прикл. биохим. и микробиол. - 2005. - Т. 41 (№ 6). - С. 681-685.Ahmed S.I., Swain T. The effect of light on the activity of enzymes of the aromatic pathway in peas and mung beans // Phytochemistry. - 1970. - V. 9. - P. 2287-2290.Ahmed S.I., Swain T. The effect of light on the activity of enzymes of the aromatic pathway in peas and mung beans // Phytochemistry. - 1970. - V. 9. - P. 2287-2290.Bradford M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding // Annu. Biochem. - 1976. - V. 72. - P. 248-254.Bradford M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding // Annu. Biochem. - 1976. - V. 72. - P. 248-254.Burda S., Oleszek W., Lee C.Y. Phenolic compounds and their changes in apples during maturation and cold storage // J. Agr. Food Chem. - 1990. - V. 38. - P. 945-948.Burda S., Oleszek W., Lee C.Y. Phenolic compounds and their changes in apples during maturation and cold storage // J. Agr. Food Chem. - 1990. - V. 38. - P. 945-948.Camm E.L., Towers G.H.N. Phenylalanine ammonia lyase // Progress in Phytochemistry [Vol. 4] / L. Reinhold, J.B. Harborne and T. Swain (eds.).- Oxford: Pergamon Press, 1977. - P. 169-188.Camm E.L., Towers G.H.N. Phenylalanine ammonia lyase // Progress in Phytochemistry [Vol. 4] / L. Reinhold, J.B. Harborne and T. Swain (eds.).- Oxford: Pergamon Press, 1977. - P. 169-188.Cheng G.W., Breen P.J. Activity of phenylalanine ammonialyase (PAL) and concentrations of anthocyanins and phenolics in developing strawberry fruit // J. Amer. Soc. Hort. Sci. - 1991. - V. 116. - P. 865869.Cheng G.W., Breen P.J. Activity of phenylalanine ammonialyase (PAL) and concentrations of anthocyanins and phenolics in developing strawberry fruit // J. Amer. Soc. Hort. Sci. - 1991. - V. 116. - P. 865869.Coseteng M.Y., Lee C.Y. Changes in apple polyphenoloxidase and polyphenol concentrations in relation to degree of browning // J. Food Sci. - 1987. - V. 52. -P. 985-989.Coseteng M.Y., Lee C.Y. Changes in apple polyphenoloxidase and polyphenol concentrations in relation to degree of browning // J. Food Sci. - 1987. - V. 52. -P. 985-989.Dangelmayr B. Relationship between flower development, anthocyanin accumulation and activity of enzymes involved in flavonoid biosynthesis in Matthiola incana R. / B. Dangelmayr, G. Stotz, R. Spribille et al. // Br. Z. Naturforsch. - 1983. - V. 38. - P. 551-555.Dangelmayr B. Relationship between flower development, anthocyanin accumulation and activity of enzymes involved in flavonoid biosynthesis in Matthiola incana R. / B. Dangelmayr, G. Stotz, R. Spribille et al. // Br. Z. Naturforsch. - 1983. - V. 38. - P. 551-555.Given N.K., Venis M.A., Grierson D. Phenylalanine ammonia-lyase activity and anthocyanin synthesis in ripening strawberry fruit // J. Plant Physiol. - 1988. - V. 133. - P. 25-30.Given N.K., Venis M.A., Grierson D. Phenylalanine ammonia-lyase activity and anthocyanin synthesis in ripening strawberry fruit // J. Plant Physiol. - 1988. - V. 133. - P. 25-30.Gulahmadov S., Kuliyeva S. and Kuliyev A. Intracellular Localization and Molecular Forms of Phenylalanine Ammonia-Lyase in Apple Fruits (Pyrus Domestica Borkh.) // Int. J. Agric. Biol. - 2013. - № 13. - P. 847-855.Gulahmadov S., Kuliyeva S. and Kuliyev A. Intracellular Localization and Molecular Forms of Phenylalanine Ammonia-Lyase in Apple Fruits (Pyrus Domestica Borkh.) // Int. J. Agric. Biol. - 2013. - № 13. - P. 847-855.Hahlbrock K., Grisebach H. Enzymic controls in the biosynthesis of lignin and flavonoids // Annu. Rev. Plant Physiol. -1979. - V. 30. - P. 105-130.Hahlbrock K., Grisebach H. Enzymic controls in the biosynthesis of lignin and flavonoids // Annu. Rev. Plant Physiol. -1979. - V. 30. - P. 105-130.Heller W., Forkmann G. Biosynthesis // The flavonoids / J.B. Harborne (ed.). - L.: Chapman and Hall, 1988. - P. 399-425.Heller W., Forkmann G. Biosynthesis // The flavonoids / J.B. Harborne (ed.). - L.: Chapman and Hall, 1988. - P. 399-425.Jiang Y., Jouce D.C. ABA effects on ethylene productions, PAL activity, antocianyn and phenolic contents of strawberry fruit // Plant Growth Regul. - 2003. - V. 39. - P. 171-174.Jiang Y., Jouce D.C. ABA effects on ethylene productions, PAL activity, antocianyn and phenolic contents of strawberry fruit // Plant Growth Regul. - 2003. - V. 39. - P. 171-174.Jones D.H. Phenylalanine ammonia-lyase. Regulation of its induction, and its role in plant development // Phytochemistry. -1984. - V. 23. - P. 1349-1359.Jones D.H. Phenylalanine ammonia-lyase. Regulation of its induction, and its role in plant development // Phytochemistry. -1984. - V. 23. - P. 1349-1359.Lister C.E. Developmental changes in the concentration and composition of flavonoids in skin of a red and a green apple cultivar / C.E. Lister, J.E. Lancaster, K.H. Sutton et al. // J. Sci. Food Agr. - 1994. - V. 64. - P. 155-161.Lister C.E. Developmental changes in the concentration and composition of flavonoids in skin of a red and a green apple cultivar / C.E. Lister, J.E. Lancaster, K.H. Sutton et al. // J. Sci. Food Agr. - 1994. - V. 64. - P. 155-161.Maier V.P., Hasegawa S. L-Phenylalanine ammonia-lyase activity and naringenin glycoside accumulation in developing grapefruit // Phytochemistry. - 1970. - V. 9. - P. 139-144.Maier V.P., Hasegawa S. L-Phenylalanine ammonia-lyase activity and naringenin glycoside accumulation in developing grapefruit // Phytochemistry. - 1970. - V. 9. - P. 139-144.Margna U. Control at the level of substrate supply - an alternative in the regulation of phenylpropanoid accumulation in plant cells // Phytochemistry. - 1977. - V. 16. - P. 419-426.Margna U. Control at the level of substrate supply - an alternative in the regulation of phenylpropanoid accumulation in plant cells // Phytochemistry. - 1977. - V. 16. - P. 419-426.Stafford H.A. Flavonoid Metabolism. -Boca Raton, Fl.: CRC Press, 1990. - 560 p.Stafford H.A. Flavonoid Metabolism. -Boca Raton, Fl.: CRC Press, 1990. - 560 p.Swain T., Williams C. The role of phenylalanine in flavonoid biosynthesis // Phytochemistry. - 1970. - V. 9. - P. 21152122.Swain T., Williams C. The role of phenylalanine in flavonoid biosynthesis // Phytochemistry. - 1970. - V. 9. - P. 21152122.Walker J.R.L. Studies on the enzymic browning of apple fruit // N.Z. J. Sci. -1962. - V. 5. - P. 316-326.Walker J.R.L. Studies on the enzymic browning of apple fruit // N.Z. J. Sci. -1962. - V. 5. - P. 316-326.Zenner K., Bopp M. Anthocyanin turnover in Sinapis alba L. // J. Plant Physiol. - 1987. - V. 126. - P. 475-482.Zenner K., Bopp M. Anthocyanin turnover in Sinapis alba L. // J. Plant Physiol. - 1987. - V. 126. - P. 475-482.Zucker M. Induction of phenylalanine deaminase by light and its relation to chlorogenic acid synthesis in potato tuber tissue // Plant Physiol. - 1965. - V. 40 (№ 5). - Р 779-784.Zucker M. Induction of phenylalanine deaminase by light and its relation to chlorogenic acid synthesis in potato tuber tissue // Plant Physiol. - 1965. - V. 40 (№ 5). - Р 779-784.

The authors declare that there are no conflicts of interest present.