geomgouГеографическая среда и живые системыGeographical Environment and Living Systems2712-76132712-7621Московский государственный областной университетgeomgou-290Research ArticleРАЗДЕЛ I. БИОЛОГИЯИЗУЧЕНИЕ БАКТЕРИОЦИНОВ ШТАММА ENTEROCOCCUS FAECALIS T23, ИЗОЛИРОВАННОГО ИЗ ИРАНСКОГО СЫРАSTUDY OF BACTERIOCINS PRODUCED BY THE STRAIN ENTEROCOCCUS FAECALIS T23 ISOLATED FROM IRANIAN CHEESEМирхади ЗадиТенназMirhadi ZadiT. .azmbi@mail.ruАбдуллаеваНармина ФуадовнаAbdullayevaN. .azmbi@mail.ruКулиевАкиф АлекберQuliyevA. .azmbi@mail.ruБакинский государственный университетРоссияBaku State UniversityRussian Federation201306052022011721Copyright © Мирхади Зади Т..., Абдуллаева Н.Ф., Кулиев А.А., 20222022Мирхади Зади Т., Абдуллаева Н.Ф., Кулиев А.А.Mirhadi Zadi T..., Abdullayeva N..., Quliyev A...Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.geoecosreda.ru/jour/article/view/290

В ходе исследования бактериоцинов, продуцируемых штаммом Enterococcus faecalis T23, установлены свойственные им особенности. Спектр антимикробной активности данного штамма определяли против различных патогенных и условно-патогенных бактерий. Штамм проявил ингибирующую активность против Listeria monocytogenes, Salmonella typhimurium и близкородственных штаммов Lactobacilli. Изучение влияния различных ферментов на антимикробную активность выявило, что активный компонент имеет белковую природу. При помощи ПЦР анализа, со специфичными праймерами для различных классов бактериоцинов, были амплифицированы два бактериоцин-кодирующих гена в геноме исследуемого штамма: entB and entA. Изучено влияние различных факторов среды, а также различных химических соединений на активность бактериоцинов. Бактериоцины проявили высокую термостабильность и сохраняли активность в широком диапазоне рН (3-10). Тритон X-20, Тритон X-80, Тритон X-100, в-меркаптоэтанол, Na-EDTA, SDS и NaCl не влияли на активность бактериоцинов.

We have partially characterized the bacteriocin activity of the strain Enterococcus faecalis T23. The antimicrobial activity of this strain was determined against Listeria monocytogenes, Salmonella typhimurium and closely related Lactobacilli strains. Treatment with different enzymes revealed that the active substance has a protein nature. PCR amplification resulted in detection of two bacteriocin genes: entB and entA. Bacteriocins of the studied strain were heat stable and active over a wide range of pH (3-10). Triton X-20, Triton X-80, Triton X-100, в-mercaptoetanol, Na-EDTA, SDS and NaCl did not influence the bacteriocin activity.

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The authors declare that there are no conflicts of interest present.